Regulatory
P-alsT

Part:BBa_K818300:Design

Designed by: Emeraldo Jo   Group: iGEM12_Groningen   (2012-09-19)

Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 465
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 465
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 465
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 465
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The suffix restriction site is PstI - SpeI instead of SpeI - PstI. This is important regarding cloning strategy.

Source

This part is from Bacillus subtilis genome.

References